Preliminary Phytochemical Screening and In Vitro Antioxidant Potential of Stem extract of Cassia fistula Linn

This study was intentional to assess the antioxidant activity of drugs viz hydro alcohol stem extract of Cassia fistula Linn. (Family: Leguminosae) is also known as Aragvadha. The primary Phytochemical study and in vitro antioxidant study was performed on hydro alcoholic extract of shade dried stem. Modern Phytochemical screening of the plant has shown the presence of phenolic compounds, fatty acids, flavonoids, tannins and glycosides. The extracts were evaluated for their phenolic content & antioxidant activity. Phenolic content was measured by using FolinCiocalteu reagent & was calculated as Gallic acid equivalents. Antiradical activity of hydro alcoholic extract was measured by DPPH (2, 2‐diphenyl‐1‐picryl hydrazyl) assay & was compared to ascorbic acid (vitamin C), and Ferric reducing power of the extract was also evaluated by Oyaizu method. In the present study three methods were used for evolution of antioxidant activity. The first two methods were for direct measurement of radical scavenging activity & remaining one method evaluated the reducing power. all the assays carried out found that the plant has antioxidant potential, These results clearly indicate that Cassia fistula are effective against free radical mediated diseases and also helpful to draw special attention for further studies.